Followed the method of Westerfield [1] to partially digest the chorion with Pronase so that the embryos fall out ofDiscussionOur development of an efficient deyolking protocol for early embryos will influence many areas of zebrafish research. Most remarkably, it enabled us to undertakePage 4 of(page number not for citation purposes)BMC Developmental Biology 2006, 6:http://www.biomedcentral.com/147