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Spondence: masanobu.takahashi.a7@tohoku.ac.jp; chikashi@tohoku.ac.jp Equal contributors 1 Department of Clinical Oncology, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan Full list of author information is available at the end of the articleother solid cancers including thyroid cancer ( 60 ), colorectal cancer ( 10 ), and lu
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A, c ?200; b, d ?Quadri et al. BMC Cancer (2017) 17:Page 5 ofFig. 3 Photomicrographs of pancreatic cancer tissue microarray (TMA) cores of p-ERK immunohistochemical staining: a no staining for p-ERK (score 0); b weak p-ERK (score 1+) staining; c moderate p-ERK (score 2+) staining; d strong p-ERK (score 3+) staining. Magnification: a-d: ?70 cases as 2+ (58.3 ), and 16 cases as 3+ (13.3 ) (Fig. 5a).
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Rtner and regulator of KSR1 and the MAPK pathway.One of the main functions of the scaffold protein CNKSR1 is the regulation of other cancer-related signaling pathways integrating output to different intracellular signaling cascades upon cellular stimulation by extracellular cues [11, 12, 17?0]. In breast and cervical cancer cell models, a pro-oncogenic potential has been demonstrated through ERK-i
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Ns including phosphorylation of select sites of CNKSR1, appears in line with our findings of a correlation between the cellular distribution of CNKSR1 and nuclear p-ERK expression levels [30].Fig. 8 Cellular distribution of CNKSR1 is associated with p-ERK expression levels in pancreatic cancer specimens. a p-ERK cellular distribution and nuclear expression levels (scored as 1+, 2+, and 3+) in 86 c
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Tcaag gacacat, SNAI2 reverse; 5-gttgcagtgagggcaagaa, GAPDH forward; 5-acccagaagactgtggatgg, GAPDH reverse; 5-cagtg agcttcccgttcag.BRAF transfectionResultsScreening of BRAF-mutant specific miRNAsHuman wild-type BRAF cDNA was prepared by PCR using the primers (forward; 5-gtggaattctgcagatataagatggcg gcgctgagcggtgg, reverse; 5-gccactgtgctggatcctttgttgctactct cctgaactctctcactc), which cover full length
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Mas. Sem Oncol 2003, 30:10?4. 4. McGirt MJ, Chaichana KL, Gathinji M, Attenello FJ, Than K, Olivi A, Weingart JD, Brem H, Quinones-Hinojosa AR: Independent association of extent of resection with survival in patients with malignant brain astrocytomas. J Neurosurg 2009, 110:156?62.Toussaint et al. Molecular Cancer 2012, 11:32 http://www.molecular-cancer.com/content/11/1/Page 11 of5.6.7. 8. 9.10.11.
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Rtant given that zebrafish as a model system in toxicology is on the rise [12]. In addition to its application in proteomics deyolking will be beneficial to other subjects such as microarray experiments targeting the gene expression pattern in the embryo proper. We have already applied the protocol successfully to such an experiment (data not shown). Therefore, the deyolking protocol will prove a
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Yolk removal. Embryos with yolk (Y) were analysed in comparison with embryos after one-step deyolking (D) or after two additional wash steps (W). A. Total protein amount per embryo as determined by DC protein assay (Bio-Rad). B. Coomassie stain (0.5 embryos loaded per lane). C. Western blot against Tubulin and MEK (0.5 embryos loaded per lane). While yolk proteins were efficiently depleted, recove