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Opathol Exp Neurol 2008, 67:456?69. Le Mercier M, Fortin S, Mathieu V, Roland I, Spiegl-Kreinecker S, Haibe-Kains B, Bontempi G, Decaestecker C, Berger W, Lefranc F, Kiss R: Galectin-1 proangiogenic and promigratory effects in the Hs683 oligodendroglioma25.26.27.28.29.30.31. 32.33.34.35.36.37.38.39.40.41.42. 43. 44.model are partly mediated through the control of BEX2 expression. Neoplasia 2009, 1
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Esults. Embryos at high stage (3 1/3 hpf), 50 epiboly (5 1/4 hpf), 70 epiboly (7 hpf) and tailbud stage (10 hpf) were deyolked, separated by SDS-gel electrophoresis and Coomassie stained (A) or blotted and immunodetected with antibodies against Tubulin (55 kD) and Moesin (78/80 kD apparent molecular weight) (B). Note that total protein amount was lower in deyolked samples, therefore more embryos
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Esults. Embryos at high stage (3 1/3 hpf), 50 epiboly (5 1/4 hpf), 70 epiboly (7 hpf) and tailbud stage (10 hpf) were deyolked, separated by SDS-gel electrophoresis and Coomassie stained (A) or blotted and immunodetected with antibodies against Tubulin (55 kD) and Moesin (78/80 kD apparent molecular weight) (B). Note that total protein amount was lower in deyolked samples, therefore more embryos
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D dish to a 1.5 ml tube filled with 1 ml deyolking buffer (1/2 Ginzburg Fish Ringer [1] without Calcium: 55 mM NaCl, 1.8 mM KCl, 1.25 mM NaHCO3) by pipetting with a narrow tip so that the yolk sac is disrupted (200 tip, Sarstedt 70.760.502). Up to 100 embryos can be transferred in a 200 volume. The embryos were shaken for 5 min at 1100 rpm to dissolve the yolk (Thermomixer, Eppendorf). Cells w
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Followed the method of Westerfield [1] to partially digest the chorion with Pronase so that the embryos fall out ofDiscussionOur development of an efficient deyolking protocol for early embryos will influence many areas of zebrafish research. Most remarkably, it enabled us to undertakePage 4 of(page number not for citation purposes)BMC Developmental Biology 2006, 6:http://www.biomedcentral.com/147
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E representation of the sequence (or a very close homolog) in thePage 3 of(page number not for citation purposes)BMC Developmental Biology 2006, 6:http://www.biomedcentral.com/1471-213X/6/searched database. Since the genome sequencing project of zebrafish is still ongoing, sequence coverage in the databases is incomplete. We therefore compared three databases in regard to the success rate in prote
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R 100 mg/kg Triphala 5 times a week. Our results are consistent with previous studies where Triphala was shown to be effective in suppressing the growth ofPage 10 of(page number not for citation purposes)BMC Cancer 2008, 8:http://www.biomedcentral.com/1471-2407/8/.' .' .' .'0 0.5 1 2 4of cells with DCF fluorescenceS (5. 7KU 7U SS 6HU16 12 8 43 53 FOHDYHGFWLQTPL treatment (hours)1 P0 73/ J PO0.'
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Ained parallel sections with a pooled IgG control. This protein-level confirmation of our microarray data gave us the impetus to pursue functional in vitro and in vivo assays with galectin-1 over-expressing GBM cells.Extracellular matrix attachmentResultsIdentification of galectin-1 as a potential mediator of glioma invasionThe quantity of RNA obtained from various xenograft tumors was highly vari